Journal: Cancer Immunology, Immunotherapy : CII

Article Title: CD137 stimulation and p38 MAPK inhibition improve reactivity in an in vitro model of glioblastoma immunotherapy

doi: 10.1007/s00262-013-1484-9

Figure Lengend Snippet: Overview of the used immunomodulatory elements. Tumor-lysate-pulsed dendritic cells were used for priming of PBMC with the aim of stimulating cytotoxic T cells (CTL) that can kill tumor cells. The simultaneous activation of helper T cells can increase the activity of these cells, whereas regulatory T cells might inhibit the activity of effector cells. Antibodies directed at CD137 can increase survival of effector T cells. Antibodies against CD25 can inhibit CD25-positive regulatory cells. The p38 MAPK inhibitor can inhibit regulatory T cells. In addition, this agent might positively affect the TH1 polarization activity of dendritic cells and inhibit apoptosis of CTL

Article Snippet: The p38 MAPK inhibitor SB203580 (Biaffin, Kassel, Germany) was dissolved in dimethyl sulfoxide (DMSO) and was used at a concentration of 1 μM.

Techniques: Activation Assay, Activity Assay

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: CD137 stimulation and p38 MAPK inhibition improve reactivity in an in vitro model of glioblastoma immunotherapy

doi: 10.1007/s00262-013-1484-9

Figure Lengend Snippet: p38MAPK inhibition antibodies increase the response of TPDC-stimulated PBMC against glioma cells. a PBMC (HLA-A2-positive) were stimulated with TPDC in the presence or absence of a p38MAPK inhibitor (p38I). After 5 and 7 days of co-culture, flow cytometry with antibodies directed at the indicated antigens was performed. b PBMC (HLA-A2-positive) were cultured with U87MG tumor-lysate-pulsed DC in the presence or absence of p38I. DMSO was used as vehicle control. After 5 and 7 days of incubation, cells were re-stimulated with tumor cells from lines U87MG, T98G, and HT1080 and interferon gamma ELISPOT analysis was performed. c After 5 and 7 days of co-culture, cytotoxic activity of primed PBMC against cell lines U87MG, T98G, and HT1080 was assessed by LDH release assay. d After 5 and 7 days of co-culture, supernatants were harvested. Interferon gamma and tumor necrosis factor were analyzed by cytometric bead array. Statistically significant differences are indicated

Article Snippet: The p38 MAPK inhibitor SB203580 (Biaffin, Kassel, Germany) was dissolved in dimethyl sulfoxide (DMSO) and was used at a concentration of 1 μM.

Techniques: Inhibition, Co-Culture Assay, Flow Cytometry, Cell Culture, Incubation, Enzyme-linked Immunospot, Activity Assay, Lactate Dehydrogenase Assay

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: CD137 stimulation and p38 MAPK inhibition improve reactivity in an in vitro model of glioblastoma immunotherapy

doi: 10.1007/s00262-013-1484-9

Figure Lengend Snippet: Combination of αCD137 antibodies and p38MAPK inhibition. a HLA-A2-positive PBMC were incubated together with U87MG tumor-lysate-pulsed DC on plates coated with αCD137 antibodies (αCD137), in the presence of p38MAPK inhibitor (p38I) or in the presence of both reagents (p38I + αCD137). Analyses were performed as described before. After 5 and 7 days of co-culture, flow cytometry with antibodies directed at the indicated antigens was performed. b After 5 and 7 days of incubation, cells were re-stimulated with tumor cells from lines U87MG, T98G, and HT1080 and interferon gamma ELISPOT analysis was performed. c After 5 and 7 days of co-culture, cytotoxic activity of primed PBMC against cell lines U87MG, T98G, and HT1080 was assessed by LDH release assay. d After 5 and 7 days of co-culture, supernatants were harvested. Interferon gamma and tumor necrosis factor were analyzed by cytometric bead array. Statistically significant differences are indicated

Article Snippet: The p38 MAPK inhibitor SB203580 (Biaffin, Kassel, Germany) was dissolved in dimethyl sulfoxide (DMSO) and was used at a concentration of 1 μM.

Techniques: Inhibition, Incubation, Co-Culture Assay, Flow Cytometry, Enzyme-linked Immunospot, Activity Assay, Lactate Dehydrogenase Assay

Journal: BMC Microbiology

Article Title: MAPK involvement in cytokine production in response to Corynebacterium pseudotuberculosis infection

doi: 10.1186/s12866-014-0230-6

Figure Lengend Snippet: Effect of MAPK pathway inhibitors MAPK p38 (Ini I), ERK 1 / 2 (Ini II) and ERK 2 (Ini III) on the production of TNF-α by splenic cells obtained from CBA mice after 30 days of infection with virulent (VD57) and attenuated (T1) C. pseudotuberculosis strains, under stimulation by secreted/excreted antigens (SeT1 and SeVD57). Each experiment was carried out ≥3 times in triplicate. * p < 0.05 with respect to stimulation by SeT1; # p < 0.05 with respect to stimulation by SeVD57.

Article Snippet: Basler et al. [ ] investigated the production of TNF-α by murine macrophages infected with M. avium subspecies paratuberculosis (pathogenic) and M. smegmatis (non-pathogenic) using MAPK p38 (SB203580) inhibitors.

Techniques: Infection

Journal: BMC Microbiology

Article Title: MAPK involvement in cytokine production in response to Corynebacterium pseudotuberculosis infection

doi: 10.1186/s12866-014-0230-6

Figure Lengend Snippet: Effect of MAPK pathway inhibitors MAPK p38 (Ini I), ERK 1 / 2 (Ini II) and ERK 2 (Inb III) on the production of TNF-α by splenic cells obtained from CBA mice after 60 days of infection with virulent (VD57) and attenuated (T1) C. pseudotuberculosis strains, under stimulation by secreted/excreted antigens (SeT1 and SeVD57). Each experiment was carried out ≥3 times in triplicate. * p < 0.05 with respect to stimulation by SeT1; # p < 0.05 with respect to stimulation by SeVD57.

Article Snippet: Basler et al. [ ] investigated the production of TNF-α by murine macrophages infected with M. avium subspecies paratuberculosis (pathogenic) and M. smegmatis (non-pathogenic) using MAPK p38 (SB203580) inhibitors.

Techniques: Infection

Journal: BMC Microbiology

Article Title: MAPK involvement in cytokine production in response to Corynebacterium pseudotuberculosis infection

doi: 10.1186/s12866-014-0230-6

Figure Lengend Snippet: Effect of MAPK pathway inhibitors MAPK p38 (Ini I), ERK 1 / 2 (Ini II) and ERK 2 (Inb III) on the production of IFN-γ by splenic cells obtained from CBA mice after 30 days of infection with virulent (VD57) and attenuated (T1) C. pseudotuberculosis strains, under stimulation by secreted/excreted antigens (SeT1 and SeVD57). Each experiment was carried out ≥3 times in triplicate.

Article Snippet: Basler et al. [ ] investigated the production of TNF-α by murine macrophages infected with M. avium subspecies paratuberculosis (pathogenic) and M. smegmatis (non-pathogenic) using MAPK p38 (SB203580) inhibitors.

Techniques: Infection

Journal: BMC Microbiology

Article Title: MAPK involvement in cytokine production in response to Corynebacterium pseudotuberculosis infection

doi: 10.1186/s12866-014-0230-6

Figure Lengend Snippet: Effect of MAPK pathway inhibitors MAPK p38 (Ini I), ERK 1 / 2 (Ini II) and ERK 2 (Inb III) on the production of IFN-γ by splenic cells obtained from CBA mice after 60 days of infection with virulent (VD57) and attenuated (T1) C. pseudotuberculosis strains, under stimulation by secreted/excreted antigens (SeT1 and SeVD57). Each experiment was carried out ≥3 times in triplicate.

Article Snippet: Basler et al. [ ] investigated the production of TNF-α by murine macrophages infected with M. avium subspecies paratuberculosis (pathogenic) and M. smegmatis (non-pathogenic) using MAPK p38 (SB203580) inhibitors.

Techniques: Infection

Journal: BMC Microbiology

Article Title: MAPK involvement in cytokine production in response to Corynebacterium pseudotuberculosis infection

doi: 10.1186/s12866-014-0230-6

Figure Lengend Snippet: Effect of MAPK pathway inhibitors MAPK p38 (Ini I), ERK 1 / 2 (Ini II) and ERK 2 (Inb III) on the production of IL-10 by splenic cells obtained from CBA mice after 30 days of infection with virulent (VD57) and attenuated (T1) C. pseudotuberculosis strains, under stimulation by secreted/excreted antigens (SeT1 and SeVD57). Each experiment was carried out ≥3 times in triplicate. * p < 0.05 with respect to stimulation by SeT1; # p < 0.05 with respect to stimulation by SeVD57.

Article Snippet: Basler et al. [ ] investigated the production of TNF-α by murine macrophages infected with M. avium subspecies paratuberculosis (pathogenic) and M. smegmatis (non-pathogenic) using MAPK p38 (SB203580) inhibitors.

Techniques: Infection

Journal: BMC Microbiology

Article Title: MAPK involvement in cytokine production in response to Corynebacterium pseudotuberculosis infection

doi: 10.1186/s12866-014-0230-6

Figure Lengend Snippet: Effect of MAPK pathway inhibitors MAPK p38 (Ini I), ERK 1 / 2 (Ini II) and ERK 2 (Inb III) on the production of IL-10 by splenic cells obtained from CBA mice after 60 days of infection with virulent (VD57) and attenuated (T1) C. pseudotuberculosis strains, under stimulation by secreted/excreted antigens (SeT1 and SeVD57). Each experiment was carried out ≥3 times in triplicate. * p < 0.05 with respect to stimulation by SeT1; # p < 0.05 with respect to stimulation by SeVD57.

Article Snippet: Basler et al. [ ] investigated the production of TNF-α by murine macrophages infected with M. avium subspecies paratuberculosis (pathogenic) and M. smegmatis (non-pathogenic) using MAPK p38 (SB203580) inhibitors.

Techniques: Infection

Journal: BMC Microbiology

Article Title: MAPK involvement in cytokine production in response to Corynebacterium pseudotuberculosis infection

doi: 10.1186/s12866-014-0230-6

Figure Lengend Snippet: Effect of MAPK pathway inhibitors MAPK p38 (Ini I), ERK 1 / 2 (Ini II) and ERK 2 (Inb III) on the production of IL-4 by splenic cells obtained from CBA mice after 30 days of infection with virulent (VD57) and attenuated (T1) C. pseudotuberculosis strains, under stimulation by secreted/excreted antigens (SeT1 and SeVD57). Each experiment was carried out ≥3 times in triplicate.

Article Snippet: Basler et al. [ ] investigated the production of TNF-α by murine macrophages infected with M. avium subspecies paratuberculosis (pathogenic) and M. smegmatis (non-pathogenic) using MAPK p38 (SB203580) inhibitors.

Techniques: Infection

Journal: BMC Microbiology

Article Title: MAPK involvement in cytokine production in response to Corynebacterium pseudotuberculosis infection

doi: 10.1186/s12866-014-0230-6

Figure Lengend Snippet: Effect of MAPK pathway inhibitors MAPK p38 (Ini I), ERK 1 / 2 (Ini II) and ERK 2 (Inb III) on the production of IL-4 by splenic cells obtained from CBA mice after 60 days of infection with virulent (VD57) and attenuated (T1) C. pseudotuberculosis strains, under stimulation by secreted/excreted antigens (SeT1 and SeVD57). Each experiment was carried out ≥3 times in triplicate. * p < 0.05 with respect to stimulation by SeT1.

Article Snippet: Basler et al. [ ] investigated the production of TNF-α by murine macrophages infected with M. avium subspecies paratuberculosis (pathogenic) and M. smegmatis (non-pathogenic) using MAPK p38 (SB203580) inhibitors.

Techniques: Infection